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Both sequences were sufficient to drive accumulation of EGFP in the nucleus, with stronger nuclear targeting observed for EGFP-NLS2.
Nonetheless, the levels of targeting observed in this study have been obtained with a minimal system that relies on simple base pairing to a guide RNA, in contrast to the ZFN and TALEN proteins, which require a new protein to be engineered for each new cleavage site.
These results indicate that the differential miRNA targeting observed between adult and neonatal naive CD8+ T cells is not complicated by 3′-UTR isoform usage and further indicate that Eomes, Tbx21, Irf1, and Il6st are likely regulated by miR-29 or miR-130 in vivo.
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Protein targets observed include chymotrypsin inhibitor II (CI2), 235) cold shock protein (CSP), 236) RNase H, 237) and LacY.
Among the ERα targets observed after 4 hour E2 stimulation of MCF7, only one target remained hormone responsive in the tamoxifen-resistant MCF7-T subline (NRF1; Figure 5).
SN group: five rabbits were submitted to a progressive SN dose infusion to reach similar pressure targets observed in the CH group (Table 1).
I used author's software, TFSVM, to evaluate overlap between algorithm's prediction of myc gene targets and measurements of 688 direct gene targets observed in (Zeller et al, 2006).
Of the expressed miRNA transcription factor targets observed, only the miR156/157 family target squamosa promoter binding protein-like 11 (SPL11) was found differentially expressed with significant (p ≤ 0.05) up-regulation at the stage two of ovule development in apomictic species.
where θRc is the nominal angle of the target observed from the receiver.
Fig. 3 Correlation between target (observed) and output (simulated) concentrations of malic acid using ANN model.
The emission of fragments from the target observed by means of quadrupole mass spectroscopy and target surface as observed by SEM were described.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com