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The targeting vector pACN-TV [11] was used for the construction of the I-Aβ-K225R-EGFP targeting construct.
The targeting construct was checked by sequencing and restriction digestion at each stage of its construction.
S.-C.J.L. designed the Kit allele targeting construct and targeted mice.
Efforts to ensure homologous recombination have necessitated engineering long flanking regions in the targeting construct.
The targeting construct was electroporated into JM8.N4 ES cells derived from C57BL/6 NTac mice and positive clones selected by G418 selection.
(A) Schematic depiction of the CD177 targeting construct.
Lanes 1 and 3: hESCs with random integration of the targeting construct.
The screening utilizes a PCR product derived from the neomycine sequence through the short arm out to the region of homology beyond the targeting construct.
The targeting construct is depicted on Figures 1A.
Details of the targeting construct are given on Figure 1A.
The targeting construct is depicted in Fig. 1a.
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