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Thus, examination of ABCA1 mediated cholesterol efflux is a plausible target to quantify efflux capacity that could provide great relevance in assessing the risk of atherosclerosis.
To investigate donor-directed B cell responses, a recent study 19 used donor-derived fibroblasts as targets to quantify DSA-secreting cells isolated from peripheral blood of KTRs before and after transplantation.
Quantitative approaches have been implemented using amoA as target to quantify specific populations of AOB in WWTP (Dionisi et al. 2002a, b) and to study the dynamics of different AOB populations overtime (Layton et al. 2005).
Future strategies aim to quantify target CPs in differently treated cell cultures and address their genotoxicity.
Quantification of the parasite load in murine tissues was done with 2 separate quantitative PCRs (QPCRs): (i) one PCR targeting kDNA to quantify Leishmania as described by Nicolas et al [22] and (ii) one PCR targeting the single copy gene neurotrophin 3 of mice to quantify the amount of murine cells present in each sample (Table 2).
To determine the effect of elevated CO2 on colonization and disease establishment by P. cajani in pigeonpea, we targeted 5.8S rDNA to quantify the fungal DNA in host tissue.
Peaks were quantified using MSD Chemstation software (Agilent Technologies, version E.02.00.493), using ion 57 as the target ion to quantify the abundance of each CHC compound.
All samples were run in duplicate along with dilutions of known amounts of target sequence to quantify the initial cDNA copy number (Concentration = EfficiencyΔCt).
A newly designed QPCR assay targeting a conserved region within the c-phycocyanin β-subunit gene (cpcB) was used along with a microcystin synthetase gene (mcyE) targeting QPCR assay to quantify the spatiotemporal patterns of total and toxigenic Microcystis.
Tracking funds targeting surgery helps to quantify and clarify current investments and funding gaps, ensures resources materialise from promises and promotes transparency within global health financing.
Computer systems used in surveillance rooms that surveillance staff use to target suspect players to quantify their threat to the house.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com