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The alignment summary presents targeted sequences accession no., species name, target length, gaps, matches and identity between query and targeted sequences.
PlasmoAP algorithm is used to predict the putative apicoplast targeted sequences [ 115].
It uses amino acid frequency and distribution to identify putative apicoplast targeted sequences.
This in silico PCR simulation allows selecting the primer pairs that only amplify the targeted sequences.
The shRNA targeted sequences were as follows: IGF1R, 5′-GCTGATGTGTACGTTCCTGAT-3′; GFP, 5′-CGTGATCTTCACCGACA AGAT-3′.
In addition, we used 1,968 sets of 16S rRNA targeted sequences [ 27] (Dataset 2) with 68,667,837 sequences and total size of 11,878.4 MB to test the performance of 16S rRNA targeted sequences analysis.
Of the four, NimbleGen and Agilent technologies have the most in common, sharing almost 40 Mb of targeted sequences.
Alternatively, another study (29) has proposed padlock-capture of targeted sequences containing known SNPs to increase read depth.
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Next, we analyzed the transcripts with extensions for features typically associated with dinoflagellate peridinin plastid-targeted sequences.
Mitoprot uses two algorithms to predict mitochondrial targeted sequences (N-terminal protein region that can support a mitochondrial targeting sequence and the cleavage site).
The statistical model assumes that the number of Pol II-targeted sequences contained within each genomic region follows a Poisson distribution.
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