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To use this to monitor mitochondrial turnover, we targeted it to mitochondria and confirmed its selective localization in the mitochondrial matrix and also confirmed that the rate of color maturation was consistent with previous reports, indicating that the matrix environment did not affect the time constant of the clock.
We therefore used also GFP for the Tubb3 transgenic line, but targeted it to a subcellular location distinct from Tis21-nucGFP, i.e. the Golgi complex/plasma membrane (Tubb3-mGFP), to be able to discern progenitor-expressed and neuron-expressed GFP.
To inactivate p53 function we targeted it to the cell membranes by post translational modifications.
No substantial effect on Tat levels was observed by MG132 treatment except for the ubiquitin-K48 mutant cotransfection group, which indicated that the lysine 48-linked ubiquitination of Tat targeted it to proteasome-dependent degradation.
The Lippard lab used a BG-conjugated version of ZP1 and targeted it to the mitochondria and Golgi with the SNAP-tag system, demonstrating the feasibility of this approach for targeting small-molecule sensors.
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Over-expressed Mdm2 protein conveys its inhibitory effect by binding directly to p53 either to inhibit its transcriptional activity or to target it to proteasomal degradation.
"We were not targeting it to be a play off a vulgar word," she added.
This is development at its best, allowing people to own and control their energy and targeting it to their needs.
I'd target it to established print-plus-digital organisations employing trained reporters to cover defined areas (say parliament, local councils, education, courts and health).
It was launched this February and the company is targeting it to be in 22 cities by the end of September.
People will share more and fundamentally different types of content, because they can target it to just their close friends.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com