Sentence examples for targeted introduction from inspiring English sources

Targeted introduction of a double-stranded break (DSB) using designer zinc finger nucleases (ZFNs) in mammalian cells greatly enhances gene targeting – homologous recombination (HR) at a chosen endogenous target gene, which otherwise is limited by low spontaneous rate of HR.

The targeted introduction of mutations utilizing sequence specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (RNA-guided nucleases, RGNs) has revolutionized reverse genetic approaches in numerous model organisms.

They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes.

All three methods are based on the targeted introduction of DNA double-strand breaks into the genome.

Notwithstanding these issues, there are specific populations that would clearly benefit from early, aggressive, and targeted introduction of insulin therapy.

The crux of this system is the efficient and targeted introduction of DNA breaks that are repaired by any of several pathways in a predictable but not entirely controllable manner.

Such targeted introductions of mutations to generate "knock-in" mutant mice, have recently been used to create models of ocular disease that closely mimic their human counterparts [19] [21].

Advising parents on how to deal with older children's non-core food consumption may benefit strategies targeting introduction of these foods.

Eucalyptus genetically engineered for freeze tolerance and targeted for introduction as a biomass crop in forest plantations in the Southeastern USA exemplifies a transgenic biofeedstock crop where the risk assessment focuses on the invasiveness of the whole plant [ 60].

In countries where such variation in burden, access and coverage are known to exist, collection of regional data and application of this type of index would help to identify regions for targeted campaign introduction, channelling resources to areas in greatest need.

We select the problem of the inventory control of minor actinides by target introduction into fast reactors.