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Follenzi A, Sabatino G, Lombardo A, Boccaccio C, Naldini L. Efficient gene delivery and targeted expression to hepatocytes in vivo by improved lentiviral vectors.
To address these limitations, targeted expression profiling methods were developed based on multiplex RT-PCR amplification followed by quantitative analysis of mRNA abundance by next-generation sequencing.
Cancer-specific promoters are useful tools to accomplish targeted expression; however, high levels of gene expression are needed to achieve therapeutic efficacy.
Reporter lines revealed that under low P conditions auxin levels are up-regulated at the root tip, whilst targeted expression studies demonstrate that auxin is mobilized via AUX1 to the root hair differentiation zone.
Because of the central nervous system involvement in DM1, we targeted expression of (CUG 60 and i CUG 480 RNA to the Drosophila MBs (Figure 5I).
All three URE2 sequences targeted expression to the telencephalon and diencephalon.
The Wu lab also targeted expression of functionally active Egfr, again using the UPII promoter (Cheng et al., 2002).
Finally, to define the subcellular localization of Sac1 in vivo, we targeted expression of Flag-Sac1 in salivary glands.
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Using MGC-targeted expression, we found that increased Ras signaling rescued the lethality associated with expression of a dominant-negative spen transgene.
Coupled with genetically-targeted expression, ChR2 affords exquisite functional control of specific neuronal subpopulations.
We then investigated whether hepatocyte-targeted expression by IDLVs induces transgene-specific immunological tolerance.
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