Sentence examples for targeted colonies from inspiring English sources

Correctly targeted colonies were verified for only a single integration event using fluorescence in situ hybridation with the purified, labelled BAC DNA.

5-Fluoroorotic acid counter-selection was used to isolate targeted colonies that were then mated with BY4742 ungΔ to generate the final BY4743 ungΔ/ungΔ YBR194W-snR6/YBR194W strain.

As 11 targeted colonies can be obtained from 3 × 10 MSCs transfected, the total cell number could be calculated as 1.1 × 10.

It should be noted that when AREG expression was targeted, colony formation significantly decreased compared with when AREG expression was not targeted in the shRNA-scrambled-transduced A431 cells.

Woo et al. [139] reported that overexpression of miR-128 in ovarian cancer cells resulted in reduced cell motility and adhesion by directly targeting colony-stimulating factor-1 (CSF-1).

Probably due to their lower cytotoxicity, TALENs were more efficient than ZFNs in generating correctly targeted iPSC colonies, but all corrected iPSC clones showed no signs of mutations at the top-ten predicted off-target sites of both nucleases.

Solid-phase assays allowed accurate staining, thus enabling the identification of target locations and the selection of target colonies under heterogeneous conditions.

In addition, probe 1 allowed solid-phase colony-based assays of K. aerogenes through the formation of insoluble colored precipitates, thus enabling accurate staining of target colonies under heterogeneous conditions.

The background bacteria were counted as non-target colonies in the total coliform analysis.

Effectiveness of each control depends on the capture rate, number of colonies targeted, size of each colony, and movement of individual cats between colonies.

Therefore, WT colonies did not produce a band, while correctly targeted clones produced a band of 1.7 kb.