Sentence examples for targeted amplified from inspiring English sources

Several molecular markers have been developed for Lupinus species, including RFLPs, ITAPs (Intron targeted amplified polymorphic sequences), and AFLPs, which have been used to build genetic linkage maps in L. albus[ 19] and L. angustifolius[ 20, 21].

Such a strategy may represent one method to target amplified transcription factors indirectly.

Fully-methylated and non-methylated DNA targets amplified from clones of these three genes were used to test the accuracy and reproducibility of the RMEAM method.

For example, for the 24 primer pairs for which the mouse was an index species, 24 of the rat and 23 of the hamster targets amplified, but only 14 of the cow, 14 of the goat, and 16 of the pig targets amplified (p ≤ 0.01 for within- vs. between-Order amplification).

Further molecular characterization of the amplified regions should facilitate the identification of the target amplified genes in CC.

Mutated and normal sequence probe oligonucleotides)about 12.7 nmol per mg nanoparticles) were used to entrap reporter fluorescein molecules inside the pores and hybridization with single stranded DNA targets amplified by PCR gave different fluorescent signals for mutated targets.

Isolated RNA was transcribed to cDNA using RETROscript™ (Ambion, Austin, TX) and cDNA targets amplified by PfuTurbo™ DNA Polymerase (Stratagene, La Jolla, CA) in a one-step reaction.

This ensured the resulting fluorescence originated from a single PCR product, and did not represent non-specific primer dimers formed during PCR or an off target amplified product.

Genomic DNA rather than a plasmid bearing a ssrRNA gene was used to generate the standard curve, because it reflects more accurately the multiple targets amplified since there are more than 5 different ssrRNA genes in the genome of Plasmodium.

Relative quantitation of the targets amplified via SYBRgreen assays was performed by the comparative ΔΔCt method.

All the cDNA targets were amplified, but only 49 of the 60 gDNA targets amplified well enough for analysis (Supplementary file 1E,F).