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(vii) The presence and prevalence of drives should be monitored by targeted amplification or metagenomic sequencing of environmental samples.
Viral genome sequencing and targeted amplification studies have led to the important discovery that phage encode environmentally relevant genes of host origin [27], [29], [30], [32], [44], [57].
We carried out targeted amplification of the ORFs by RT-PCR and sequenced the products (before and after the cloning) to verify the ORF structures.
The prevalence of gene drives in the environment could in principle be monitored by targeted amplification or metagenomic sequencing of environmental samples.
16S rRNA gene profiling involves targeted amplification and subsequent next-generation sequencing of phylogenetically informative marker sequences of microbial DNA [ 20].
On the basis of microscopic examination of paraffin embedded tissues, molecular tests for fungal pathogens were performed by a ribosomal internal transcribed spacer targeted amplification reaction.
Similar(47)
PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA.
The most familiar formats use DNA or RNA target amplification methods for enhanced sensitivity above culture and antigen-based procedures.
Our marker design targeted the amplification of intron-rich nuclear single-copy regions from genomic DNA.
To confirm the specific target amplification, the real-time amplification was followed by melting curve analysis from 65°C to 95°C.
Despite widespread use, multiplex PCR is still baffled by inherent problems such as preferential target amplification and nonspecific amplifications.
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