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In A. thaliana, miRNAs may target transcripts that encode proteins and transcription factors [ 43].
They regulate gene expression by triggering sequence-specific cleavage or translational repression of target transcripts.
MicroRNAs (miRNAs) are small regulatory non-coding RNAs that repress target transcripts post-transcriptionally.
Then, the target transcripts were cleaved by the AGO1 complexes in the cytoplasm.
MicroRNAs are 20 22 nucleotide RNAs which bind to target transcripts and regulate gene expression.
This approach identified known and previously unknown miR-155 target transcripts.
Heterologous hybridisation requires microarray probes that are capable of tolerating a certain degree of mismatch with the target transcripts.
The 3′ cleavage remnants from the target transcripts are relatively stable in vivo, and could be detected by sequencing.
Using published algorithms to predict target transcripts for these miRNAs, we identified putative target transcripts of the 63-set, which were in turn subjected to pathway analysis.
The miR528 and miR535 sequences identified were used in other species to search for target transcripts, but no similar target transcripts could be identified.
However, no target transcripts that could be involved in flowering were identified.
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