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Thus, examination of ABCA1 mediated cholesterol efflux is a plausible target to quantify efflux capacity that could provide great relevance in assessing the risk of atherosclerosis.
Quantitative approaches have been implemented using amoA as target to quantify specific populations of AOB in WWTP (Dionisi et al. 2002a, b) and to study the dynamics of different AOB populations overtime (Layton et al. 2005).
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To investigate donor-directed B cell responses, a recent study 19 used donor-derived fibroblasts as targets to quantify DSA-secreting cells isolated from peripheral blood of KTRs before and after transplantation.
Future strategies aim to quantify target CPs in differently treated cell cultures and address their genotoxicity.
Peaks were quantified using MSD Chemstation software (Agilent Technologies, version E.02.00.493), using ion 57 as the target ion to quantify the abundance of each CHC compound.
All samples were run in duplicate along with dilutions of known amounts of target sequence to quantify the initial cDNA copy number (Concentration = EfficiencyΔCt).
Quantification of the parasite load in murine tissues was done with 2 separate quantitative PCRs (QPCRs): (i) one PCR targeting kDNA to quantify Leishmania as described by Nicolas et al [22] and (ii) one PCR targeting the single copy gene neurotrophin 3 of mice to quantify the amount of murine cells present in each sample (Table 2).
Each PCR amplification was performed in triplicate wells under the following conditions: 94°C for 10 min, followed by 40 or 45 cycles at 94°C for 15 s and 60°C for 1 min. cDNA plasmid standards, consisting of purified plasmid DNA specific for each individual target, were used to quantify the target gene in the unknown samples, as described [ 29].
Computer systems used in surveillance rooms that surveillance staff use to target suspect players to quantify their threat to the house.
For example, an extended drug-target network including the experimental medicines (drugs in the pipeline or not yet approved by the FDA) for CVD therapy and their therapeutic targets are used to quantify trends in exploitation of novel drugs and targets (see details in Additional file 1 ).
The DNA sample representing the target to be quantified reached the interval of the generated curve among the points from the dilution series.
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