Sentence examples similar to target to amplify from inspiring English sources

Two pairs of nested primers, targeted to amplify the swamp buffalo SRY conserved region, were designed for sex identification.

A 648 bp fragment of gyrA covering QRDR was targeted to amplify by PCR in 18 selected isolates.

The effect outcome is inhibited action of Amelogenin gene primer that is targeted to amplify Amel gene of particular sex in order to determine sex of the volunteers.

Control primers (set 6, Table  3) were designed against SRY, presumed to be single copy, targeted to amplify from all these five species.

We designed 2,138 EST-SSR primer pairs from the identified SSRs (Additional file 2), with 94.6% of them targeted to amplify perfect repeats and over 70% of the primers targeted at trinucleotide motif types.

We designed a set of primers targeted to amplify a 111 base pair sequence located in the upstream region of the RASSF1A promoter (ranging from -135 to -24 base pairs from the starting site of exon 1) (Table 2).

Indeed Lee et al. [ 17] designed a set of primers targeted to amplify a sequence of exon 1 of the gene and not of its promoter, as we did.

In the absence of exonucleolytic digestion, it is most likely that each target molecule only hybridizes with a single CP molecule, with no subsequent target recycling to amplify the reaction.

In the first step a pair of PCR primers targeting sequences within the 3'UTR (UTR-primed PCR) was designed for each of the target genes to amplify a fragment of about 400 bp (see methods).

The goal of the first PCR (PCR#1) is target enrichment to amplify regions of interest from bisulfite-converted gDNA.

The resulted products were subjected to two cycles of PCR with adaptor targeted primers to amplify the desired differentially expressed sequences.