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Exome and target sequencing studies have shown to be an effective tool for the discovery of new ASD genes.
This provided an excellent opportunity to compare the inter-laboratory reliability using two different target sequencing methods and sequencing platforms.
The goal of this pilot study was to develop a customized, cost-effective amplicon panel (Ampliseq) for target sequencing in a cohort of patients with sporadic phaeochromocytoma/paraganglioma.
Due to the weaknesses of target sequencing and metagenomic sequencing, single-cell sequencing is becoming a powerful complementary approach, which aims at sequencing target bacteria at single cell levels.
The applications of sequencing technologies on environmental microbiology can be mainly divided into target sequencing, metagenomic sequencing and single-cell genomic sequencing, according to the problems they need to be solved.
Target sequencing also called amplicon sequencing, which sequence specific marker genes of microbes such as 16S ribosomal RNA (16S rRNA), ITS, ammonia monooxygenase subunit A gene, or methyl-coenzyme M reductase alpha subunit gene, etc.
Similar(30)
The target sequence is underlined.
gRNA target sequences are also shown.
The filtered sequences were termed target sequences (tags).
The yellow shadow marks the target sequence recognized by crRNA.
A selected target sequence contained AA leader followed 19-nt.
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