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Following a target scan of miR-183, we identified 242 conserved targets, including molecules crucial for the development of pancreatic beta-cells such as Foxo1.
Focusing on the 2 miRNA common to exosomes derived from all 5 RMS cell lines miR-12466 and miR-1268), we employed Target Scan software to identify their putative targets.
Using the Target Scan software and PANTHER analysis, we identified 4395 putative targets of miR-1246 and miR-1268 combined, with effects on pathways implicated in tumorigenesis, such as Wnt, Cadherin, EGF and FGF, angiogenesis, and apoptosis signaling.
Target Scan version 7.0 (http://www.targetscan.org) was used to predict gene targets of the 2 common deregulated miRNA identified, and Panther Software (http://pantherdb.org) was used to identify the biological pathways linked to these targets.
Recording data for image normalisation: white target scan and dark image scan.
Figure 18 shows the STFT for the no target scan and Figure 19 shows STFT for target case.
Very little overlap was found between the predicted (Pictar and Target Scan) and experimental targets.
Target Scan 3.0 and PicTar were used to predict gene targets of the deregulated miRNAs identified in this study.
Surprisingly, Target Scan did not predict any targets for miR-1538 nor are there any published reports on this miRNA.
Table 2 lists the top 3 predicted targets according to both Target Scan and PicTar for each differentially expressed miRNAs.
Each age-associated miRNA was tested using Target Scan, which uses a computational algorithm to identify predicted mRNA targets.
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