Antigens were retrieved using the Dako Target Retrieval solution in conjunction with steam.
To stain for F4/80-positivity, antigens were retrieved using the Dako target retrieval solution (Dako, Carpinteria, CA).
Epitopes were retrieved at high temperature in Dako Target Retrieval Solution; slides were then cooled and rinsed in distilled water.
High temperature target retrieval.
This design enabled us to separate lateralized activity related to target retrieval from distractor processing.
For immunohistochemical analysis, sections were placed on positively charged glass slides and treated with EnVision™ FLEX Target Retrieval Solution (high pH) using a PT-Link (Dako, Denmark).
Sections were rehydrated and antigen retrieval was performed using the PT Link pre-treatment (Dako) with Target Retrieval Solution, pH 6 (Dako), preheated to 65°CC.
Briefly, sections were deparaffinized and treated with Target Retrieval Solution (Dako) according to the manufacturer's protocol.
Formalin-fixed sections (human section) were, then, treated with target retrieval solution (DAKO, Carpinteria, CA) for 10 min in steamer; no target retrieval treatment for zinc-based Beckstead's solution-fixed mouse tissues.
Antigen retrieval was performed for 2 minutes in preheated target retrieval solution (Dako) using a pressure cooker.
Antigen retrieval was performed for 90 s in preheated target retrieval solution (Dako) using a pressure cooker.
