Initial understanding of miRNA-mRNA target recognition came from observations of sequence complementarity between lin-4 and multiple conserved sites within the gene lin-14 3′-UTR [ 35].
Toxicity of RNAi can occur through improper target recognition resulting from suboptimal annealing of the antisense guide strand to the target strand, improper selection of an unintended passenger strand by the RISC complex, or overloading of the silencing machinery.
From three F1 clutches, we sequenced the TRα TALEN target recognition site region from three individuals from each clutch with a normal and mutant phenotype (comprising a total of 79 successful sequencing runs after TOPO cloning) (Supplemental Table 2).
Proof-of-principle that PA substitution need not prevent target recognition was obtained from studies using PPIP5K2.
The PRI-lock probes, combined with the OpenArray™ system, offer a flexible and adaptable design of high-throughput, quantitative multiplex detection assays, since the target recognition is separated from further downstream processing.
This insertion acts as "a molecular toggle" and provides a unique target recognition property that differs from that of authentic Pax-61.
Recent work in the computational modeling of visual attention has demonstrated that a purely bottom-up approach to identifying salient regions within an image can be successfully applied to diverse and practical problems from target recognition to the placement of advertisement.
This indicates that some information important for miRNA target recognition is still missing from the prediction algorithms.
Moreover, the fluorescence responses of 12 depicted in Figure 3C demonstrate the expected functionality for target recognition and for discrimination from near-cognate sequences.
Since it contains the target structure signatures, such as target size and scatterer distribution, radar HRRP target recognition has received intensive attention from the radar automatic target recognition (RATR) community [1 16].
