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As another way of understanding the magnitude of loss, this lost seafood could provide the total yearly target quantity of protein for 10.1 million men or 12.4 million women, EPA + DHA for 20.1 million adults, and calories for 1.5 million adults.
The target quantity of pendrin was determined from the standard curve and divided by the target quantity of β-actin RNA for normalisation.
Target quantity of unknowns was interpolated from given the CT of any unknown.
Relative transcript levels were determined from the relative standard curve constructed from stock cDNA dilutions, and divided by the target quantity of the calibrator following the manufacturer's instructions.
It was expected that the infant would consume the initial target quantity of 15 g lyophilized beef/day by 7 months of age.
For genes which were not amplified with the same efficiency as the endogenous control, The Relative Standard Curve method in which target quantity is determined from the standard curve and divided by the target quantity of the calibrator was used.
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Target quantities of all other samples were expressed as n-fold in relation to the calibrator.
Relative quantity (RQ) was obtained by dividing the target quantity with the quantity of the endogenous control in each sample.
In contrast to plateau drift that can generate underestimations of Emax, inclusion of collapsed cycles overestimates Emax, which in turn generates an underestimation of target quantity.
Another important aspect revealed by this dataset is a progressive underestimation of target quantity as Emax is reduced, as reflected by the predicted F0 (Figure 4).
The CT was used for kinetic analysis and was proportional to the initial number of target quantity copies in the sample.
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CEO of Professional Science Editing for Scientists @ prosciediting.com