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Such knockout strategies can support further improvements of the target productivity.
The results demonstrated the strategies to improve target productivity by gene disruption; some of them could not be achieved by previous screening methods.
For this purpose, constraint-based metabolic flux simulation is a powerful tool to predict the effect of reaction knockouts on target productivity.
The metabolome and flux distribution of the metabolic pathways enable us to evaluate the metabolic state and provide useful clues to improve target productivity.
Iterative screening for increasing the target productivity can be carried out at relatively low computational costs.
Our PLS model was able to capture most of the variance in the target productivity, despite using only 3 latent variables to describe the input data).
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Knockout sets leading to higher target productivities can effectively prune suboptimal solutions, thereby reducing computation time to identify the global optimal solution in comprehensive screenings (Kim et al., 2011).
Mark Pemberthy, director of JLT employee benefits, said: "It is ironic that in an autumn statement which is targeting productivity, some of the incentives that employers use to raise productivity and engage their workforce have been made less affordable".
Model driven software engineering (MDSE) is an emerging methodology for software development, targeting productivity, flexibility and reliability of systems; metamodelling is at the core of most MDSE approaches.
Therefore, iterative screening using is insufficient for identifying the reaction knockouts that cause high-target productivity.
A longer exposure period significantly decreased the dry matter, viability, and productivity of target products by using fungi (Sulaiman et al. 2011).
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