Sentence examples for target preparations from inspiring English sources

In our study however, both target preparations were prepared using oligo dT) primers for the synthesis of first strand cDNA, so this explanation is less likely.

For further verification of the transcript profiling results, selected genes were analyzed by quantitative real time RT-PCR with cDNAs prepared from the identical RNA samples used for microarray target preparations.

To evaluate cross-hybridization of rRNA in the target preparations, we performed subtractive hybridization assays.

We observed high reproducibility (R2≥0.96), for target preparations from the same RNA in two independent reverse transcription reactions, or from two different freshwater samples collected at the same location at approximately the same time (Fig. S2B).

Thus, an increased proportion of mRNA in the target preparations resulted in an increase in signal intensity, indicating that our probes hybridized specifically with corresponding mRNA-derived targets (and did not cross-hybridize with rRNA).

Material from the 21 target preparations was then hybridized to MOE430A chips.

Target preparation of biological samples by the FIB technique especially offers the possibility of preparing not only soft materials but also hybrid samples (soft/hard materials).

Fig. 9 Impact of the protein target preparation.

Herein we broadly review fluorescence-based assay formats with a focus on PCR-associated target preparation.

At the same time, this production pathway is accompanied by a comparatively less complex target preparation and separation procedure.

44CaCO3, 97.0 % enriched (Trace Sciences International, USA) and graphite powder, 99.9999 % (Alfa Aesar, Germany) were used for target preparation.