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Herein we broadly review fluorescence-based assay formats with a focus on PCR-associated target preparation.
The abundance of 5′ ITS cRNA fragments produced during target preparation was sufficient to drive undesirable hybridization.
Fig. 9 Impact of the protein target preparation.
At the same time, this production pathway is accompanied by a comparatively less complex target preparation and separation procedure.
44CaCO3, 97.0 % enriched (Trace Sciences International, USA) and graphite powder, 99.9999 % (Alfa Aesar, Germany) were used for target preparation.
It was determined that higher 43Sc activities were accessible via the 43Ca production route, with a comparatively less complex target preparation and separation procedure.
Target preparation and array hybridization were performed as previously described [70], [71].
Both target preparation and hybridization were performed in the Microarray Core Facility, University of Kentucky.
More details on biological specimen collection and processing, target preparation, scanning, and genotype generation are provided in [8].
A similar approach was used to evaluate variability introduced by sampling and target preparation protocols (Fig. S2B).
Target preparation, microarray hybridization, post-hybridization processing and scanning, and normalization were done essentially as described [13].
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