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Target predictions were run for all predicted miRNAs.
Interestingly, 38% of these predicted disruptions were identified in 8mer target predictions.
The conventional in silico target prediction approach [10] is extended in this study by using protein domain annotations to extrapolate from non-plasmodial target predictions to protein target predictions in P. falciparum.
TargetScanS [28], PITA[29] and picTar[30] target predictions are obtained from the respectively internet sites.
miRNA target predictions were obtained from TargetScanMouse 5.0 (http://www.targetscan.org/mmu_50/).org/mmu_50/
We downloaded Pictar miRNA target predictions [33] from the UCSC genome browser [34].
The miRBase Targets Release Version v5 is comprised of gene target predictions for 711 human miRNAs.
Target predictions for miR-199a-5p miR-199a-5p miR-199a-5pfor C11Orf9 represhowedinteractions.
We downloaded PITA miRNA target predictions and the PITA executable [5] from http://genie.weizmann.ac.il/pubs/mir07/index.html and miRNA target predictions from Stark et al. [7] from http://compbio.mit.edu/fly/motif-instances.
From http://www.mirz.unibas.ch/ElMMo2 we extracted miRNA target predictions generated based on the algorithm previously described [58].
The integration of new genome sequences, miRNA target predictions and expression data into prediction pipelines will lead to further improvements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com