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These results show the added benefit of incorporating domain-based extrapolation for target prediction purposes.
To increase the stringency of the target prediction protocol, we searched for mRNAs simultaneously predicted by five or more different target-prediction programs.
We identified predicted target genes of miR-218 using four online miRNA target prediction algorithms.
Target prediction for plant microRNAs.
Fig. 3 Target prediction and intersection.
Graphical abstract Proteochemometric modelling coupled to in silico target prediction.
Figure 4 Complementarity between in silico target prediction and PCM.
Additional file 1: Training set for the target prediction algorithm.
The target prediction algorithm was implemented in Perl.
Bioinformaticians have applied these algorithms for theoretical miRNA target prediction.
Thus, psRNATarget and Small RNA Target Prediction should be the efficient and easy-to-use tools for plant sRNA target prediction.
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