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Because this seed sequence plays an important role to mRNA target specificity, miRNA family members are thought to have extensive mRNA target overlap and redundant functions.
Furthermore, immunoblot analysis of PUM1 and PUM2 RIP eluates with α-PUM2 and α-PUM1 antibodies, respectively, did not show co-immunoprecipitation of the two paralogous proteins, thus excluding the possibility that the target overlap was simply due to simultaneous protein pull-down (Figure S1B).
This is particularly the case for Ste12 and Cse4 which have highly dissimilar binding patterns and almost no target overlap.
(3) By means of applying target overlap scoring, many condition-specific MRANs are obtained as undirected weighted graph of miRNAs.
As a result, the list of cooperative miRNAs and the target overlap scores were used to construct CMN.
Target overlap score between a pair of miRNAs is defined as Jaccard similarity coefficient, representing the fraction of shared condition specific targets.
Similar(34)
The only pan-ebolavirus neutralizing Abs reported thus far target overlapping epitopes in the viral IFL21,23,24,25,26.
Furthermore, the IVT tracker may also fail when the target overlaps with a similar object.
Though these adhesins target overlapping binding sites on the non-glycosylated face of the aminoterminal IgV-like domain, they are structurally highly diverse [50], [51], [52].
Interestingly, a more continuous analysis of target overlaps based on pair-wise global comparisons of each data set (Figure 2A C) suggested that the occupancy of target promoters by Myc was largely consistent across cell lines and growth conditions.
(A ) The Sal target overlaps the RNAP active-center region.
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