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Finding one small molecule (query) in a large target library is a challenging task in computational chemistry.
Microwave irradiation promoted 3 out of the 4 steps to establish an extremely rapid, convergent and highly efficient route to the target library.
At this level of sampling it would not be feasible to screen a target library against a bait library since the diversity of clones in such an experiment (tens of millions) would greatly exceed the screening capacity of the automation.
The target library was amplified using limited cycles of PCR.
The target library was eluted from the beads into 22 μl of water.
Since the target library was designed, three more causative genes have been added to the RP gene list.
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All too often, local governments target libraries for budget cuts at the expense of the mission of providing accessible education and literacy to the public.
As with other target library-based arrays, probe designs must be updated periodically to remain current with novel genomes in GenBank.
4) PCR amplification of captured target libraries.
Prepared target libraries were sequenced using Illumina's MiSeq Desktop Sequencer.
In these samples, an average of 1.8 million reads (9.1 %; range: 4.8 %-16.72 %) per sample aligned to the target libraries before filtering the human genome.
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