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As shown before, the mtDNA sequences generated by the different capture target kits showed distinct patterns of per-base coverage depth, whereas sequences from the same capture targets largely followed the same coverage pattern but at varying read depths.
Exome sequences were generated using three different capture target kits (SureSelect Human All Exon 50Mb Kit (Agilent), TruSeq Exome Enrichment Kit 62Mb (Illumina), or SeqCap EZ Exome Library v2.0 (Nimblegen)) according to the manufacturers' protocols.
Also, comparison of different Salmonella qPCR detection kits demonstrated that single target kits were unable to detect S. bongori or an atypical S. enterica enterica Senftenberg lacking the entire Pathogenicity Island I (Hu et al. 2008) (Table 5).
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Biotin labeling of target RNAs was performed with one-cycle target kit (One-Cycle Target Labeling and Control Reagents, Affymetrix, P/N 900493), and hybridized according to the manufacturer's protocol.
In particular, miRNA-221 and miRNA-222 target KIT, an oncogene, and therefore function as tumor suppressors in erythroblastic cells and other human solid tumors (Croce 2009).
Dasatinib showed significant growth reduction on long-term coculture assay, particularly in the absence of GM-CSF, suggesting that dasatinib can target KIT mutation effectively.
Perhaps the association of decreased miR-222 levels with poor chemotherapy outcome is through its upregulation of angiogenesis via its target KIT, a tyrosine kinase receptor.
MicroRNAs that may have an essential role in GIST pathogenesis were identified, in particular miR-17/20a/222 that target KIT and ETV1.
As many FLT3-inhibitors also target KIT, AKN-028 was tested in a human megakaryoblastic leukemia cell line overexpressing KIT, in which the substance induced a significant inhibition of KIT autophosphorylation.
The concomitant increase in the expression of the two miRNAs that target KIT [ 18, 39] and the decrease in KIT expression in our malignant FNA samples strengthens the choice to use these markers in the diagnosis of nodules.
5 μg RNA were processed into biotin-labeled cRNA and hybridized to Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays (Affymetrix, High Wycombe, U.K ., using the Affymetrix One-Cycle Labeling and Control (Target) kit according to the manufacturer's instructions.
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