Sentence examples for target integration from inspiring English sources
Plasmids were digested with BstEII to target integration to LEU2, and the number of copies of MPS3 integrated was determined by Southern blotting.
The usage of a simulation environment preliminary to a waveform integration paves the way to target integration.
piggyBac has many desirable features, including seamless excision of transposons from the genomic DNA and the potential to target integration events to desired DNA sequences.
In this study, a plasmid was designed to target integration of a gfp-bsd gene into the Babesia bovis ef-1α locus.
Availability of the transient transfection system was critical for the further development of a stable transfection technique using a plasmid designed to target integration of a gfp-bsd gene into the B. bovis ef-1α locus.
It is true that the differences of the simulation environment and the real operating environment need adjustment but the simulation environment step allows breaking down the overall integration in two levels: a) the initial integration to check the functional behavior and the interfacing between software components developed independently and b) target integration with real-time constraints.
HEases have been designed to target disease-associated genes, such as XPC [ 7] and RAG1 [ 8], to induce targeted integration in cell lines [ 9, 10], and for targeted mutagenesis in crop [ 11].
Both vectors integrate into the same locus (AOX1), and therefore by using Δku70, which prevents off-target integration, a higher proportion of clones with the correct integration should be observed (Naatsaari et al. 2014).
The host cell-encoded lens epithelium-derived growth factor (LEDGF/p75) binds lentiviral integrase and targets integration to active transcription units.
The addition of the CBX1 chromodomain to MLV INW390A efficiently targeted integration away from gene regulatory elements.
Meanwhile, the Mosdi1 R left flank and right flank, which will facilitate targeted integration of foreign DNA into wild type Mosdi1 locus, was introduced in the vector.
