We studied all combinations of curated elements, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability.
In all panels, the grey band corresponds to ±5% of the target expression level.
A comprehensive understanding of the parameters that determine the relationship between microRNA (miRNA) and target expression levels is lacking.
For cell-based assays, recent reports highlight the drive towards primary cell types, endogenous target expression and label-free systems3,4,5,6.
Asundi, J. et al. MAPK pathway inhibition enhances the efficacy of an anti endothelin B receptor drug conjugate by inducing target expression in melanoma.
Pluripotency genes engaged in both "fully-reprogrammed" and "persistent-NPC" interactions exhibit over/undershooting of target expression levels in iPSCs.
While examining the predicted targets of miR-92a-3p, we noticed an interesting relationship between the level of target expression and the likelihood of the target being negatively correlated with the miRNA.
Loss of T cell function under these conditions is mediated by the microRNAs miR-101 and miR-26a, which target expression of the methytransferase EZH2 and thereby diminish the expression of anti-tumor cytokines.
Approaches to the prediction of targets of miRNAs are addressed by Nikolaus Rajewsky (p S8), who considers the case for combinatorial control of target expression by multiple miRNAs acting synergistically.
Relative quantification determines the target expression relative to an external standard or reference sample and should be adjusted for the PCR efficiencies actually achieved.
This promoter is sufficient to faithfully target expression of a reporter gene to the postmeiotic male germ cells of transgenic mice.
