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It seems a reasonable premise that dosing for targeted therapies should be driven by target effect rather than toxicity.
Subjects were studied during placebo and midazolam at 30 ng.mL -1) target effect site concentration.mL -1
Figure 4: Target effect on pupil dilations evoked by heightened vigilance.
Problem is, multiple sites in a genome can have the same series of letters, so CRISPR could snip the wrong section known as an "off target" effect.
The target effect was also clearly demonstrated by the increase in European and non-European breeders with increasing island size.
The mean amplitudes were measured between 200 and 300 ms for the N2d (i.e., the N2 target effect) and 300 and 500 ms for the P3d (i.e., the P3 target effect), respectively.
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After a 15-min control period, propofol was infused to an initial target effect-site concentration of 0.0 microg/ml with N2O or 1.5 microg/ml with air.
Subsequently, the propofol target effect-site concentration was increased by a designated increment (0.25 and 0.5 microg/ml) and the process repeated.
Our preliminary results indicated that patients did not respond to command when the target effect-site concentration was 0.19 ng/ml, but did respond when the target effect-site concentration was 0.14 ng/ml.
Evaluation of the cytotoxicity and off-target effect of iExosomes.
Besides, the potential off-target effect of RNAi in eukaryotes is also a matter of concern.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com