This scenario would imply that alteration of hair form, mammary structure and any possible effect on tooth morphology, are 'off-target' consequences of pleiotropic EDAR action.
To further confirm that the observed apoptosis in our experiments was caused by specific downregulation of MMP-2 and not due to any off-target consequences, we supplemented cells with recombinant human MMP-2 (rhMMP-2).
A vital assumption in siRNA-mediated gene silencing is that knockdown of the target gene is exclusive, and without off-target consequences.
It can be envisaged how Hfq acts as a catalyst for such recognition in vivo, but the question naturally arises how sRNAs generally achieve precision, accuracy, and speed in producing their effects and avoid undesired off-target consequences.
More complete studies are needed to determine the role of MRJ-L in macrophages, as well as more directed therapeutic targeting to specific cells to avoid potentially devastating off-target consequences.
In particular, the choice of the target has consequences on the induced radioactivity and dose rates in the target itself and in its surroundings.
Epigenetic regulation of gene expression and function via altering histone/DNA methylation and acetylation levels and their therapeutic targeting is currently a rapidly developing field and will likely provide new opportunities to target the consequences of metabolic reprograming in tumors and potentially immune cells.
A performance-based framework was built for the safety design of chemical facility layout, which contains four key components: hazard identification, performance target confirmation, consequence assessment and defending effect evaluation.
If you recommend military action, you've got to think, What forces, what targets, what consequences?
If a particular gene-drive sequence mutates, it could end up affecting different targets, with consequences that would be hard to predict.
Revenues are also below target, a consequence of the continued failure to crack down on tax evasion.
