Sentence examples for target complementarity from inspiring English sources
The Streptococcus pyogenes Cas9 nuclease (Cas9) cleaves the intervening spacer sequence directed by a single guide RNA (gRNA) with a 20 nucleotides (nt) target complementarity region, at its 5′ end.
Finally, the automiG sensor is also self-silenced by the anti-GFP miRNAs in HeLa cells and might be easily used to identify factors involved in miRNA biogenesis and silencing guided by perfect target complementarity in mammals.
Further, any sequence can be placed in between the two regions of target complementarity within each ligamer.
It depends on target complementarity, 3′-end modification, cellular condition, and extracellular signaling (Katoh et al. 2009; Rüegger and Großhans 2012).
A-to-I editing can either affect the biogenesis of miRNAs [ 16- 18] or the specificity of miRNA:target complementarity [ 19].
They can either be cis-encoded antisense RNAs, and thus fully complementary to their targets, or trans-encoded at a distant location with only partial target complementarity [ 5].
Probes were designed to minimize off-target complementarity using NCBI's BLAST Danio rerio RefSeq RNA database.
The method considers the 3′UTR regions of the transcriptome and calculates context score [ 41] from the type of seed match (8mer, 7mer-m8, and 7mer-1A), 3′ pairing contribution (miRNA-target complementarity outside the seed region); preferentially conserved targeting (PCT) estimated as described in [ 42] and [ 43]; and multiple additional parameters.
Where we were able to predict D and D' box sequences with reasonable certainty based on positioning and similarity to the consensus, predictions were generally considered significant if the guide-target complementarity contained at least nine consecutive canonical Watson-Crick base pairs and encompassed the plus five nucleotide methylation site (for exceptions to this general rule, see below).
The asterisk indicates a mismatch base pair at the target site in the region of guide-target complementarity The pan-archaeal analysis of experimentally verified C/D box sRNA molecules was used to deduce 2'- O-methylation patterns for archaeal rRNA molecules.
Highlighted bases in the alignment are as follows: (green) D guide predictions; (blue) D′ guide predictions; (magenta) sites that are predicted by more than one sRNA guide; (red) predictions that contain a mismatch base pair at the site of modification in the region of guide-target complementarity.
