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The mirror-image kissing complex was successfully formed when the L-AVP target bound to the engineered L-aptaswitch element.
This potentiating effect determined increased FA variations between the duplex and target bound states of the aptameric probe.
The DNA sandwiched target bound on the magnetic beads is then separated by using a magneto electrode based on graphite epoxy composite.
In the presence of NF-κB p50, the triplex became unstable since the target bound to the recognition sequences with strong affinity.
The assay was based on the AuNPs difference in affinity for single-stranded DNA (non-binding) and double stranded DNA (target bound).
For each of these categorizations a given target may be in more than one group, for example a Tup1 target bound by both Sut1 and Mig1.
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The labeled target solution is then hybridized to the chip where target binds to probe DNA.
This set of kinases is only a fraction of the intracellular targets bound by the degrader, demonstrating that successful degradation requires more than target engagement.
In particular, it was found in in vitro experiments that the death rate of targets in vitro bound by 2 CTL is not dramatically different from death rate of targets bound by 3 or 4 CTLs [42].
We interpret these targets to be TPP-specific protein targets bound in vivo in mouse liver.
Sephton et al. (2011) identified RNA targets bound by TDP-43 in vivo by RIP-seq.
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