Sentence examples for target bead from inspiring English sources

By contrast with this last case, LFA-1 engagement did not generate any pushing forces on the target bead.

As for engagement of TCR-CD3 alone, the pushing-pulling sequence ended in target bead engulfment by the T cell.

Hence, engagement of LFA-1 alone triggered neither pushing force nor engulfment of the target bead, although small pulling activity and restricted calcium signaling were detected.

To this purpose the BFP-held target bead was this time coated with LFA-1 ligands (anti-CD18 antibodies to the β2 chain of LFA-1) and brought into contact with the T cell just as described above with TCR-CD3 ligands.

In order to determine the initial pulling force loading rate, r = dF/dt, developed by the T cell against the target bead, the force transducer elongation was measured as a function of time in the initial stage of this pulling phase for a given probe stiffness equal to 50 pN/µm.

Each sample was deposited on a quadrant of the slide at a target bead density of 60-70 k beads/panel.

The screening was terminated till the significant (>1000) selectivity of the phages to the target beads was achieved.

Quantification was done with the instrument software and the number of copies of DNA selected with target beads and (His 6 beads were compared to determine signal/background ratios.

After the first two selection rounds, the final two of five target beads washes were done for 5 minutes instead of 30 seconds.

The phages recovered from both of the HVGGSSV peptide-coated beads and the control beads were also titrated; selectivity of the phages to the HVGGSSV peptide was estimated with the ratio of the phages recovered from the target beads to those recovered from the control beads.

Eventually, it is no longer feasible to identify the target beads from the overwhelming background, regardless of how much laser power is applied.