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To understand the EET and trapping processes in more detail, a target analysis was used to fit all datasets simultaneously.
Figure 1: Experiment timeline and target analysis.
It is called Operational Target Analysis and dated January 2010.
Dai, X. & Zhao, P. X. psRNATarget: a plant small RNA target analysis server.
Wang, S. et al. Target analysis by integration of transcriptome and ChIP-seq data with BETA.
The target analysis species is human by default, since human-centric databases are often the most comprehensive.
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Off-target analysis of sgRNA2b-induced human ES cells.
The whole genome sequence dataset used for off-target analysis can be accessed at EGA (EGAS00001003436).
Extended Data Fig. 6 Off-target analysis of human CD34+ HSPCs edited by SpCas9 RNP targeting BCL11A enhancer.
J.H.H. designed the research, performed PACE, characterized variants in bacteria, conducted human cell experiments, analysed data, performed off-target analysis and wrote the manuscript.
Off-target analysis was performed following a previous study with modifications (Liang et al., 2015).
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