Sentence examples for target amplified from inspiring English sources

Further molecular characterization of the amplified regions should facilitate the identification of the target amplified genes in CC.

This ensured the resulting fluorescence originated from a single PCR product, and did not represent non-specific primer dimers formed during PCR or an off target amplified product.

Such a strategy may represent one method to target amplified transcription factors indirectly.

A recent study, however, had implicated fibroblast growth factor receptor 2 (FGFR2) as novel therapeutic target amplified in TNBCs [ 59].

The result is obtained by detection of a cleaved dual fluorescent dye-labelled oligonucleotide detection probe that permits the detection of C. trachomatis target amplified product (amplicon) and C. trachomatis Internal Control DNA, which is amplified and detected simultaneously with the specimen.

For every target, amplified PCR products of 10 random positive samples were separated by agarose gel electrophoresis to control for purity and size of the amplicons.

Mutated and normal sequence probe oligonucleotides)about 12.7 nmol per mg nanoparticles) were used to entrap reporter fluorescein molecules inside the pores and hybridization with single stranded DNA targets amplified by PCR gave different fluorescent signals for mutated targets.

Isolated RNA was transcribed to cDNA using RETROscript™ (Ambion, Austin, TX) and cDNA targets amplified by PfuTurbo™ DNA Polymerase (Stratagene, La Jolla, CA) in a one-step reaction.

Genomic DNA rather than a plasmid bearing a ssrRNA gene was used to generate the standard curve, because it reflects more accurately the multiple targets amplified since there are more than 5 different ssrRNA genes in the genome of Plasmodium.

Relative quantitation of the targets amplified via SYBRgreen assays was performed by the comparative ΔΔCt method.

All the cDNA targets were amplified, but only 49 of the 60 gDNA targets amplified well enough for analysis (Supplementary file 1E,F).