This study targets a SON ANS functionality, which determines the most suitable access network from a user QoS point of view.
A target branching functionality of around 15 side chains per backbone chain and a constant branch molecular weight of either 5000 g mol−1 (seriesries) or 30 000 g mol−1 (seriesries) were used for each generation.
While the first generation does not display a target specific functionality to bind exclusively to one (or few) targets, the second generation allows target specific binding.
Some of these antibodies are not confirmed as blocking antibodies and hence there is a possibility that they do not target the functionality of the protein in an optimal way.
For example, in a study of epistatic interactions in A. thaliana, [ 33] discovered a recent asymmetric gene duplication for which the target retained functionality in one strain while the source was the functional copy in another strain.
These can be proteolytic degradation, inhibition of target functionality and target production [ 2, 3].
We next annotated each hit for target site functionality, by checking the literature source for evidence of a loss of function experiment, which provided us with 606 validated MRE targets.
This process usually begins with the specification of a desired target structure or functionality.
If this hypothesis of a conserved role for MAPK signalling is correct, there must be convergence of the pathway in mice and humans at the level of MAP2K, MAPK or target protein functionality and this can be tested by the identification of sex reversing MAP2K or MAPK mutations in both mice and humans, or mutations in MAPK target genes.
On the other hand, controls using isotype IgG did not show any effect (data not shown), suggesting that the specific antibodies interacted with target protein functionality.
However sophisticated, sequence considerations cannot eliminate all peroxisomal false positives as target sequence functionality has been shown to be dependent on numerous factors, including protein conformation and targeting sequence accessibility.
