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In this study, using a Solexa sequencing technology, small RNAs in developing radish taproot from three different developmental stages: pre-cortex splitting stage (Stage1, 10 DAS), cortex splitting stage (Stage2, 20 DAS) and expanding stage (Stage3, 40 DAS), which cover the key morphological changes that occur during the taproot thickening process, were firstly isolated.
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As a result, some known and new miRNA families were isolated from these three taproot libraries, from which the differentially-expressed miRNAs involved in taproot thickening were identified.
Secondary roots (<1 mm diameter) were pulled from the main taproot randomly from across the root system until approximately 10 mg of tissue had been collected.
Total RNA of the three taproot samples from different stages was isolated using the RNAprep pure Plant Kit (Tiangen Biotech Co., Ltd., China) according to the manufacturer's protocol.
Habit: Annual from taproot or perennial herb from rhizome; hairs branched or not, glandular or not.
For the coarse lateral roots, two discs were also taken, one from the insertion point on the taproot and another from the middle of it.
Habit: Annual to perennial herb, generally glandular-hairy, taprooted or from +- thick caudex.
Habit: Biennial from taproot.
Habit: Annual from taproot.
Habit: Annual, from taproot.
Habit: Generally biennial from taproot.
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CEO of Professional Science Editing for Scientists @ prosciediting.com