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Tris-Acetate-Phosphate (TAP) plate and liquid medium was used for culturing the cells.
6. Tap plates on paper to remove excess liquid in-between washing steps.
Then the UV-mutagenized cells were spread on TAP plates and incubated in the dark until colonies appeared.
Transformed cells were plated onto fresh TAP plates containing 15 µg/ml zeocin (Z) and placed in the dark at 25°C.
Serial dilutions of WT and sak1 were spotted onto minimal (HS) plates at the indicated light intensity or on TAP plates containing the indicated inhibitor.
Cells were pretreated with 1 μM RB with or without light, then spotted on minimal plates and grown under high light (HL) or grown photoheterotrophically on TAP plates containing norflurazon (NF) and grown under low light for 4 days.
A total of 5500 colonies were picked, transferred onto fresh TAP plates and maintained at low light (LL, 15 μmol photons m-2 s-1) for 5 to 10 days.
Agbayani did, eluding the tag of catcher Chris Widger and tapping the plate with his left hand.
You believe that tapping a plate is going to make you feel a lot better, and it does, so it's reality".
When tapping the plate, the animals moving in a continuous coordinated sinusoidal way were classified as fast movement.
We first arrested cells at the prometaphase by Nocodazole treatment, and the arrested cells were collected by tapping the plate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com