Exact(9)
Tandem affinity purification (TAP) analysis was modified from Nachury's method (Nachury, 2008).
The RAP and TAP analysis at the three codon positions reveals interesting contrasts between the two.
This TAP analysis identified all known IFT complex B proteins, but no IFT complex A proteins nor motor proteins.
The RAP and TAP analysis in this study may provide guidance for further bioinformatic and genetic investigations into the underlying principles for these mutation and selection forces.
In the latter Tap Analysis Programme, householders can volunteer to test their own tapwater using easy-to-use kits and then receive individualised results once samples have been processed by an appropriate laboratory [ 18].
TAP analysis and co-purification experiments with the different point mutants of Arh1 and Yah1 should help in clarifying if Arh1 and Yah1 act in tandem during ISC biogenesis.
Similar(51)
We limited the analysis to 40 out of initially 80 proteins determined by SVD, since the remaining proteins were not detected in the wild-type Rpd3-TAP analysis.
For example, TAP-analysis of SCRIB, a protein important in the development of cell polarity, was used to identify a protein complex that is associated with the metastatic progression of breast cancer [ 71].
Patients with positive Borrelia antibody test were asked to undergo a spinal tap with analysis for intrathecal Borrelia antibody production.
This strategy led to identification of 5 additional components (Ssu72p, YOR179C, C1p1p, Pcf11p, Rna15p) which were also found in TAP-purification analysis [ 45].
We next used Pathway Palette (Askenazi et al. 2010) to query Wormbase and retrieve experimentally based genetic, physical and regulatory interactions among the 20 proteins identified in our TAP-MS analysis.
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