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TAP-1 knockout mice show significantly reduced levels of MHC class I surface expression [31].
TAP-1 and TPN increased over 23 and 20 times respectively, compared to undifferentiated Shef-1 cells.
Regarding the APM genes, TAP-1, TPN, LMP2 and RFX5 mRNA levels plummeted during the reprogramming process to iPSCs.
We found normal expression levels of TAP-1 and Tapasin but significant down-regulation of TAP-2 in OE19.
Similarly, the NT2 cell line showed the highest up-regulation in HLA-B, β2m, TAP-1 and TPN genes.
RT-PCR and IHC was performed on EAC biopsies to check as well the expression of TAP-1 and Tapasin.
To determine the effector specificity of the C-terminal portion of Tap-1, V52Δ tap-1 was provided with episomal tap-1 V52 or tap-1 AM−19226.
Tap-1 is encoded upstream of tseL.
A 1587Δ tap-1 mutant was provided with episomal tap-1AM−192262 or tap-1.
We hypothesized that tap-1 contains a recombination site to create a diverse Tap-1 family.
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