Sentence examples for tank transfer from inspiring English sources

Subsequently, separated proteins were transferred using a Hoefer TE 22 tank transfer unit (Amersham Biosciences) onto Immobilon-P PVDF membrane (Millipore) in fresh transfer buffer (25 mM Tris, 192 mM glycine, 10% methanol, 0.1% SDS).

Denaturing was done at 95°C for 5 min and was followed by electrophoresis at 100 V for 1 h and 50 min. The separated proteins were transferred to a Polyvinylidene fluoride membrane (0.45 µm) in a tank transfer system with transfer buffer (25 mM Tris(hydroxymethyl -aminomethan (Tris), 192 mM glycine, 20% methydroxymethyl -aminomethan V for 2 h.

SDS-PAGE gels were transferred via standard wet tank transfer to PVDF membranes.

Proteins were transferred to PVDF membrane (Bio-Rad) using a tank transfer system (Bio-Rad) with Towbin buffer without methanol [ 38].

The Mini-PROTEAN electrophoresis system combined with the Mini-Trans Blot module (Bio-Rad, Hercules, CA, USA) was used for protein electrophoresis and tank transfer.

Proteins were electroblotted onto hydrophobic polyvinylidene difluoride membranes (Immobilon, Millipore, Milan, Italy) using a tank transfer system (Bio-Rad Laboratories Srl).

The same or similar individual tanks were used for conducting the experimental procedures, therefore maintaining a constant environment throughout the experimental period and reducing the stress associated with the between-tank transfer.

Following the completion of the electrophoresis, the proteins were transferred to 0.45 μm nitrocellulose (Whatman) using a wet-tank transfer with 25 mM Tris, 192 mM glycine and 10% methanol as the transfer buffer.

For example, carbon steel was found to be susceptible to stress corrosion cracking (SCC) when exposed to fuel-grade ethanol (Kane et al. 2005; Lou et al. 2010; Lou et al. 2009; Sridhar et al. 2006), and exposure of steels to fuel-grade ethanol has resulted in failures of storage tanks, transfer piping, and other equipment (Kane et al. 2004).

This unit existed until 1975, when it was disbanded and its tanks transferred to the Uad-Ras Mechanized Infantry Regiment. Uad-Ras Mechanized Infantry Regiment

Proteins were separated on SDS PAGE gels and transferred to a polyvinylidene difluoride membrane in a wet tank using transfer buffer for 1.5 h [ 78].