Recombinant Reg IV protein was purified by tangential flow filtration and reverse phase chromatography.
A tangential flow filtration system was then used to concentrate the prepared nanosuspension.
In this situation, a tangential flow filtration system (Millipore, Billerica, MA, USA) was used to exchange the preparation into PBS.
Section 1 discusses non-chromatography methods, including precipitation, liquid liquid extraction, and high performance tangential flow filtration.
The extraliposomal buffer was exchanged with 10-mM HEPES-buffered saline (HBS, pH 6.5) via tangential flow filtration (Spectrum Labs; Rancho Dominguez, CA, USA).
This work describes a shortcut approach for the design of tangential flow filtration for the concentration of human induced pluripotent stem cells (hiPSC), supported by design of experiments.
Here, we compare SCD with the more traditionally used Tangential Flow Filtration (TFF), and demonstrate the physicochemical and biological comparability of LNPs produced with both methods.
In this study a two-step tangential flow filtration (TFF) process using hollow fibers was developed for the clarification and concentration of HIV gag-VLPs.
For this larger preparation, a tangential flow filtration system with a 50 kD MWCO was used to eliminate the LMW species, exchange the buffer, and to also concentrate the F ab' 2.
A first ultrafiltration (UF) step was performed on a tangential flow filtration system (Sartoflow Alpha crossflow system, Sartorius) by using a polyethersulfone membrane (0.1 m2) with a cut-off of 100 kDa.
Therefore, we first developed a tangential flow filtration (TFF) method, a highly effective purification method for lyophobic colloids [20], to quickly purify the protein of any amino group-bearing by-products which might compromise reaction efficacy.
