Sentence examples for tangential flow filter from inspiring English sources
The tangential flow filter used was a VivaFlow 200 cassette (Sartorius) composed of polyethersulfone (PES) with a nominal pore rating of 10000 MWCO and a surface area of 200 cm200
Protease N (IUB 3.4.24.28, Bacillus subtilis) enzyme was used to continuously hydrolyse an initial 5% (w/v) whey protein isolate (86.98%, Kjeldahl nitrogen × 6.38) for 5 h at pH 7.0 and 55 °C in a 10 kDa tangential flow filter (TFF) enzymatic membrane reactor (EMR).
The fate of Protease N (IUB 3.4.24.28, Bacillus subtilis) enzyme was monitored while being used to hydrolyse an initial concentration of 5% (w/v) whey protein isolate (86.98% Kjeldahl nitrogen × 6.38) continuously for 5 h at pH 7.0 and 55 °C in an enzymatic membrane reactor (EMR) fitted with a 10 kDa tangential flow filter (TFF).
The filtrate was then concentrated to ∼500 ml on a 100 kDa tangential flow filter (TFF), which captured the unicellular eukaryotes, microbes and VLPs (i.e., the water was removed).
Metagenomes were prepared by concentrating approximately 60 l of seawater using a tangential flow filter (tff).
The environmental genomic sequences collected from Chula Vista solar salterns (Chula Vista, CA), were obtained from biomass retained on a 0.2 μm pore size tangential flow filter and were sequenced by pyrosequencing on a GS20 sequencing platform (454 Life Sciences, CT, USA).
In both cases, the filtrates were then concentrated by 0.2 µm tangential flow filters (Amersham, CFP-2-E-4A).
In addition, 100 K and 300 K tangential-flow filter (TFF) capsules were tested for filtrate concentration; the 100 K TFF capsule was more effective at removing host cell proteins for downstream virus purification (data not shown).
We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda.
At each sampling site, 180 l of samples were collected and pre-filtered through a 5.0 μm SMWP membrane (diameter 290 mm; Millipore, Ireland) to remove suspended particles, then filter-concentrated with Tangential Flow Filtration (TFF) system through Durapore 0.1 μm PVDF filter (Pellicon 2 Cassette Filter, Screen type C, size 0.5 m, Millipore Corporation, MA, USA).
A tangential flow filtration (TFF) system (Pellicon 2 Filter Acrylic Holder, Millipore, US) was used in order to filter-concentrate the samples.
