Next, the formation of autophagosomes and autolysosomes was visualised using a Premo autophagy tandem sensor kit.
To analyse NA-induced autophagosome and autolysosome formation over time in MCF-7, MDA-MB-231, and MCF-12A cells, we used the commercially available Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit (Life technologies, Carlsbad, CA, USA).
Furthermore, higher accuracy will be achieved through the use of additional GFP-based biosensors for autophagy, including 2xFYVE [ 54], pH-sensitive tandem sensors for quantifying transitions between autophagosomes and autolysosomes [ 26, 55], and single-cell, spatio-temporally correlated autophagy and apoptosis data-sets (e.g. [ 56]).
To investigate what causes the increased DR5 GFP response in zll-1, we first monitored expression of the tandem auxin signaling sensor R2D2 [ 26], where the pRPS5a:DII-n3xVenus (DII-Venus) signal is inversely correlated to auxin signaling and where the pRPS5a:mDII-ntdTomato (mDII-ntdTomato) signal acts as transcription control.
The Zfas system works in tandem with ultrasonic sensors, radar and cameras to capture driving data.
It presents a 360-degree live image of its surrounding to the 360 Eye's computer brain, telling it where to go next, and building a constantly updated model of the cleaning environment in tandem with infrared sensors.
The problem, thus far, the pair argue, is that companies like Google and Uber are throwing an engineering mindset at making vehicles autonomous, in the sense of designing rule-based systems that try to pre-empt and deal with every edge case, whilst in tandem adding more sensors and capturing more data.
The digital camera was used in tandem with the LiDAR sensor.
Second, lipid peroxidation sensor and tandem mass spectrometry experiments argue that the major diet-derived PUFA, linoleic acid, is less vulnerable to peroxidation in lipid droplet TAGs than in membrane phospholipids.
The Goldee device works using various sensors in tandem, and then sending that data to the startup for processing.
Its water sensor operates in tandem with an accompanying valve that will cut the flow as soon as a leak is detected.
