Sentence examples for tandem probe from inspiring English sources
These results were confirmed using the tandem probe RFP-GFP-LC3.
By using the mRFP-GFP-LC3B tandem probe, we discriminated between newly formed autophagosomes and autophagolysosomes, showing that STK38 depletion does not negatively impact autophagosome-lysosome fusion.
The effects of Tv6 on the autophagy flux was studied by two different methods: LC3II quantification and by using a RFP/GFP LC3 tandem probe that allows the microscopic visualization of autophagosomes and autolysosomes.
To test whether STK38 may also have a role in subsequent autophagy events like the fusion between autophagosomes and lysosomes, we used the mRFP-GFP-LC3B tandem probe [ 25].
Our data show that the farmers had consistently higher frequencies of chromosome aberrations, as determined by the standard chromosome aberration assay, and significantly abnormal DNA repair responses (p < 0.05), as determined by the challenge assay, but no statistically significant differences in the tandem-probe fluorescence in situ hybridization (FISH) assay (p > 0.05).
For microRNA expression evaluation, we used miRMAX microarray technology [ 22] that features tandem dimer probes complementary to mature microRNA sequences (or abridged sequence) of all the identified miRNAs of Drosophila according to the miRNA registry VERSION 5.1 [ 23].
Total RNA isolated from the tumour cell line (CD18/HPAF), tumour tissues, and normal human pancreas was fractionated on agarose gel electrophoresis, Northern blotted, and probed with a MUC4 tandem repeat cDNA probe.
A two-probe tandem DNA hybridization assay including capture DNA1, probe DNA2, and target DNA3 was prepared.
A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides.
PtdIns3 P is enriched on endosomal membranes and can be detected using a tandem FYVE domain probe from Hrs, which labels scattered cytosolic puncta [ 25].
A two-probe tandem DNA hybridization assay was designed for the detection of Enterobacter cloacae based on time-resolved fluorescence.
