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Retroviral vectors integrate preferentially in genes and in one-half of the integrations, the expression cassette will be in a tandem orientation with respect to the gene's promoter and potentially expressed when the trapped promoter is intrinsically active or activated by regulatory elements within the vector.
Furthermore, a block of approximately 13 kb comprising genes Os06g04690, Os06g04699 and one helitron was found duplicated in tandem orientation in Nipponbare but not in 93-11 (Figure 4 B).
An analysis of the PGS nucleotide sequence of KT71T revealed a total of 15 putative PpsR binding sites, of which 12 were arranged in a typical tandem orientation separated by seven or eight nucleotides (Figure S5).
The genes GJB2 and GJB6 are localized on chromosome 13q11-12 in tandem orientation.
R-st contains various r1 genes, four of which are in tandem orientation [ 45].
This is in contrast to the tandem orientation of Gsx and Xlox in chordates [ 56].
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We compare expression of induced genes arranged in convergent, divergent, or tandem orientations.
The CTD repeat motif and tandem repeat orientation are observed in most eukaryotic model organisms, and this configuration evolved early in the history of eukaryotes [ 16].
Circular permutated yellow fluorescent protein variants revealed that in the tandem constructs the orientation of the transition dipole moment influences the FRET efficiency.
We provide initial evidences that bidirectional promoter and transcriptional read-through might in part explain antisense expression in genomic loci with tandem and convergent orientation, respectively.
A single segmental duplication in tandem and direct orientation was detected.
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