The two derivatives of a SynE, SynE- L and SynE- L, consisted of 3 tandem copieSynE- Rch 12 N core and were generated by annealing two complementary oligonucleotideSynE- R bases in length.
In addition, hardly any SSOGs that are located in clusters (∼0.5%) appear to be tandem duplicates of each other, that is, the clusters of SSOGs typically consist of unrelated genes.
The PGD model required that after tandem duplication of each of the 13 genes, the 13 extra copies were moved to new locations and placed in the same order (and relative orientations) as their progenitors.
Two main scenarios have been put forward to account for their presence: evolutionary remnants of chromosomal "en bloc" duplications or genome duplication, followed by gene loss and inversions [ 16, 17]; or they reflect independent tandem duplications of each gene family followed by adaptive groupings of genes on different chromosomes [ 18].
Three tandem copies of each CPT1A putative PAX3-FKHR binding site, either wild-type (ie, PRO1, PRO2, PRO3, and PRO4) or with the corresponding PD site mutated (ie, PRO1m, PRO2m, PRO3m, and PRO4m), were placed upstream of a minimal thymidine kinase (tk) promoter [ 12] in the pGL4.20 reporter construct (Promega, Madison, WI).
The 338 AA Acropora predicted protein consists of an N-terminal signal peptide of 23 AA, the bulk of the rest of the protein consisting of tandem repeats, each of around 30 AA and containing two di-Cysteine motifs.
The Sounders were led by the striker tandem of Fredy Montero and Steve Zakuani, each with 10 goals.
Each repeat is a tandem of two similar α/β sub-domains, joined by a shared β-strand.
Varying in tandem each of the sector-specific estimates of the percentage of revenue spent for BIR yields a plausible range for total 2012 BIR costs of $330 billion - $597 billion (Additional file 1: Table S4).
But there will be fewer after the interim forward tandem of Fabian Espindola and Chris Rolfe were dynamic and combined for a goal each against Houston mid-week.
