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A gap here refers to a stretch of DNA for which the exact nucleotide base identity (A, G, T, or C) remain ambiguous after resequencing, represented by a tandem number of "N" between the determined sequences.
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The integrated transgene head-to-tail tandem copy number was estimated by slot blot from 1st generation offspring and ranged from 2 to approximately 200 (supplementary Table S1).
The RCCX region is a complex, multiallelic, tandem copy number variation (CNV).
The tandem repeat number at the MIRU 20 should be 2 instead of 1.
Repeats were detected with REputer [22] using default parameters and variations in tandem repeat numbers were characterized with Tandem Repeat Finder [23] and CLC genomics workbench.
VNTR: Variable number tandem repeat; MLV: Multilocus variable-number tandem repeat; MLVA: MultiLocus variable-number tandem repeat analysis; SLV: Single locus variant; DLV: Double locus variant; TLV: Triple locus variant; CLSI: Central Laboratory Standards Institute AMBB: Amphotericin B; ITZ: Itraconazole; VRZ: Voriconazole; CAS: Caspofungin; ECV: Epidemiological cut-off values.
Table 1 Variable number tandem repeats A variable number tandem repeat occurs in DNA when a pattern of one or more nucleotides is repeated, and the repetitions are directly adjacent to each other.
VNTR: Variable number tandem repeats analysis; MLVA: Multiple locus variable number tandem repeats analysis.
*VNTR, variable number tandem repeats; MIRU, mycobacterium interspersed repetitive units, ETR, exact tandem repeats.
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The variable regions, known as VNTR's, for Variable Number Tandem Repeats, are the type of differences used for human DNA fingerprinting.
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