Meanwhile, significant fluorescence changes (up to 74 77 nm) were observed during their tandem photoluminescence experiments in cyclohexane/THF and THF/water systems.
For tandem MS experiments, the system was operated in data-dependent acquisition mode with automatic switching between MS and MS/MS.
The low MS ion abundances for saturated disaccharides preclude tandem MS experiments used for differentiation of disaccharides that occur as isomer pairs.
The steady state analysis in tandem with experiments demonstrated how a complex signaling network interlinks various pathways to respond to changes in the nutritional status.
For tandem MS experiments, the system was operated with automatic switching between MS and MS/MS modes (MS 0.5 s/scan on m/z range [250 1500] and MS/MS 0.7 s/scan on m/z range [50 2000]).
The Ion Charge Control was 100000 and the maximum accumulation time was 200 ms. A total of 4 scans were averaged to obtain a MS spectrum and the rolling average was 2. For tandem MS experiments, the system was operated with automatic switching between MS and MS/MS modes.
Initial peptide identification was performed via tandem MS/MS experiments.
Sequences of the N-terminal peptides were confirmed by tandem MS experiments.
Tandem MS experiments revealed that the cluster was bound to hRPC39.
However, the low detection limits and the possibility to conduct tandem MS experiments make it an attractive approach.
