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The tertiary organization of GG-SpaD is a tandem assembly of three Ig-like domains, wherein a middle domain (M-domain) joins together the N- and C-terminal regions (N- and C-domains) of the pilin subunit (Fig. 1a, b).
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In order to acquire images from the cortex, we used a CCD camera (Adimec 1000 m, Adimec, The Netherlands) mounted on a tandem lens assembly composed of a 50 mm Nikon bottom lens adapted on a 135 mm Nikon top lens for a final magnification of ×2.7.
Genome-wide analysis of tandem gene assemblies.
We explore two cases of multicomponent interactions, H4/H6 nanostars and H4/amyloid peptide tandem plate assemblies.
In many studies on genome assembly, tandem repeats are preliminarily removed, representing a negligible fraction of the genome and facilitating the assembly procedure (see e.g., for the sunflower genome, Staton et al. 2012).
The structure of conventional tandem DSC is an assembly of two independent DSCs.
In this region we found the nitrogenase genes nifHDK next to several tandem genes important for assembly of the nitrogenase MoFe cofactor (fdxD, C1499, nifE, nifN and nifX).
These data support a model in which MDA5 uses long dsRNA as a signaling platform to cooperatively assemble the core filament, which in turn promotes stochastic assembly of the tandem CARD oligomers for signaling.
Tandem repeats in the genome assembly was identified using TRF49 (version 4.0.4).
De novo assembly often collapses tandem repeats and can also erroneously expand single-copy sequence (Salzberg et al. 2011).
Three PETs that were shown to ambiguously map to multiple genomic loci and six PETs that did not return any result, indicating that their halftags were not present in tandem on the mm6 genome assembly, were subsequently eliminated.
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